Jun 6, 2020 · ADE-OPI-MS Instrumentation. The ADE-OPI-MS instrument used in this study is schematically shown in Figure Figure1 1.The ADE technology delivers nanoliter volumes of samples with high accuracy and precision via fine-tuned acoustic energy, and it has been widely used in drug discovery for compound and sample management applications that support various medicinal chemistry, pharmacology, and ADME

Jun 26, 2022 · Abstract. Proton transfer reaction-mass spectrometry (PTR-MS) is a quantitative analytical technique for the online detection of gas-phase volatile organic compounds (VOCs), among them aroma compounds, over a broad dynamic range and down to ultra-trace concentrations. Its rapid detection capabilities and high sensitivity make it an ideal tool

Oct 29, 2014 · For large-scale and standardized applications in mass spectrometry- (MS-) based proteomics automation of each step is essential. Here we present high-throughput sample preparation solutions for balancing the speed of current MS-acquisitions and the time needed for analytical workup of body fluids. The discussed workflows reduce body fluid sample complexity and apply for both bottom-up

Nov 11, 2020 · In this review, we will discuss the prosand cons of chromatography-free MS and discuss some of the currentlyused and future technologies being investigated to enable high-throughputMS. Keywords: Mass spectrometry, ionization methods, high-throughputscreening, drug discovery.

Feb 11, 2023 · Three different chromatographs are shown in Figure 2.5.2 2.5. 2. The top one is the total ion chromatograph at the window range of 400 Da. It’s impossible to distinguish the target molecules in this chromatograph. The middle one is at one Da resolution, which is the resolution of single quadrupole mass spectrometer.

Performance Level 1: Everyday applications; choose these for the cost-effective choice; Performance Level 2: High throughput applications; choose these when robustness and reproducibility are key; Performance Level 3: High performance; choose these when sensitivity is a must; No HPLC/UHPLC or GC instrument has been left behind.

Jul 1, 2019 · We provide practical recommendations through critical reviews of high-throughput mass spectrometry options and application case studies. High-throughput experimentation (HTE) is the simultaneous execution of a large number of experimental conditions. It is usually conducted in 96-, 384- or 1536-well microtiter plates, which has the significant

Figure 1.17: Fast atom bombardment (FAB) mass spectrometry, also known as liquid secondary ion mass spectrometry (LSIMS). Fast atom bombardment is a soft ionization source which requires the use of a direct insertion probe for sample introduction, and a beam of Xe neutral atoms or Cs + ions to sputter the sample and matrix from the direct

Sep 1, 2021 · Affinity selection mass spectrometry (ASMS) has emerged as a powerful high-throughput screening tool used in drug discovery to identify novel ligands against therapeutic targets. This report describes the first high-throughput screen using a novel self-assembled monolayer desorption ionization (SAMDI)–ASMS methodology to reveal ligands for

Aug 3, 2020 · Acoustic droplet ejection mass spectrometry (ADE-MS) has recently emerged as a promising label-free, MS-based readout method for high throughput screening (HTS) campaigns in early pharmaceutical

Jul 5, 2022 · The mass spectrometer was equipped with the electrospray ionization and operated in the positive ion mode to monitor the m/z transitions for all peptides and their internal standards. Mass spectrometry parameters such as declustering potential and collision energy were manually optimized for every single peptide and were summarized in Table 1

Oct 4, 2013 · Reproducible and quantitative gas chromatography–mass spectrometry (GC-MS)-based metabolomics analysis of complex biological mixtures requires robust and broad-spectrum derivatization. We have evaluated derivatization of complex metabolite mixtures using trimethylsilyl cyanide (TMSCN) and the most commonly used silylation reagent N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA). For the

Jun 19, 2021 · We move away from light and embrace the detection of mass in the articles from Simon et al. 13 and Scholle et al. 14 showing the high potential of mass spectrometry (MS)-based applications in HTS

May 23, 2020 · A very innovative approach combining acoustic ejection and direct ionization for high-throughput mass spectrometry (HTMS) analysis has been recently developed by a group from AstraZeneca [72,73]. Dubbed acoustic mist ionization mass spectrometry, this approach uses a modified Echo acoustic dispenser to eject a “mist” of picoliter-sized

Oct 29, 2018 · Young, M. M. et al. High throughput protein fold identification by using experimental constraints derived from intramolecular cross-links and mass spectrometry. Proc. Natl Acad. Sci. USA 97 , 5802