Nov 10, 2022 · Vials. Use only Aijiren vials, the shape, and the material is optimized for maximizing instrument precision. Do not overfill the vials. Fill the vial up to 1/2 to 2/3 of the total volume. Experimental conditions. Make sure that the same solvents were used between the runs. Keep the room temperature constant and under 25 degrees Celsius.

Sep 13, 2012 · Liquid Chromatography Vials, Caps & Septa Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques. 8 posts Page 1 of 1 Vials, Caps & Septa hems Posts: 2 Joined: Thu Sep 13, 2012 4:02 am by hems » Thu Sep 13, 2012 4:18 am

PTFE/silicone septa are always best. What force is required to penetrate the septum? Generally, silicone septa are more easily pierced than red rubber or butyl septa. For thicker, hard-to-pierce septa, a small gauge (23 gauge) needle may be required, or a stronger S-needle can be used. Preslit septa are the best option for

Baseline problems arise from many causes. Some of these are: • Electronic or mechanical failure • Contamination in critical areas, such as detectors • Incorrect or inappropriate setpoints • Leaks, column or septum bleed, or other chromatographic difficulties These problems may interact to a certain degree and or arise from any of the above

Jul 23, 2020 · By definition, According to the definition, Septa is a circular gasket made of Silicone or silica gel. In HPLC analysis, Septa is usually placed in the Cap of the HPLC Vial to seal the HPLC Vial to ensure that there is no gap between the Cap and the Vial.Aijiren's Septa uses a double-sided material, and the combination of PTFE and Silicone

We have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head.From my little experience i learnt that it may be due to air trapped in

Mar 30, 2010 · The problem we're encountering is %RSD failing for linearity and accuracy preparations. Criteria is set at 10% RSD, which is pretty large, yet %RSD values are all over the place. Linearity is from 2.5 ug/mL to 1000 ug/mL. Vials for each level are prepared individually, with internal std added (125 ug/mL isobutanol).

Oct 19, 2015 · The first OOS occurred when a chemist used the caps with non-cut septa on the Shimadzu HPLC. All vials had the first injections with significantly lower areas than the following injections. Prior to this OOS, we allocated the caps with non-cut septa for the Dionex HPLC, and the caps with pre-cut septa for the Shimadzu HPLC.

Alliance HT HPLC Systems. This vial delivers maximum sample capacity (~1.5 mL) with minimum residual volume. The 9 mm cap makes it ideal for use with Aijiren HPLC and GC Systems. Choosing the level of certified vial should be based on the concentration of analyte. Concentration of analytes is often tied closely with the detection source used.

Dec 1, 2016 · First, remove the column and replace it with a piece of connecting tubing. Then replace all solvents (wash solvents and any mobile-phase solvents) with acetonitrile. Flush 10–20 mL of acetonitrile through each line to ensure all the normal-phase solvents have been flushed from the system.

Jul 25, 2015 · Abstract. "Carry-over" is a term used to describe a type of sample contamination which causes sample peaks to re-appear in later runs which do not actually contain the sample (e.g. blank runs

Jun 2, 2020 · Jun. 2nd, 2020. HPLC Sample Vial is widely used in Laboratory analysis. Food safety, Environmental monitoring, and HPLC Sample Vials take an important role. This article focuses on the application of glass sample vials for LC and LC/MS and small molecule applications. Please choose a compatible HPLC Sample Vial according to your experiment

III-3. Cyclic noise – Detector related problems and others IV- Changes in chromatography The most common changes in chromatographic responses are related to the shape and separation of peaks, their elution times and changes in established performance. The evaluation stage is best preformed on a standard rather than sample

If noise disappears, clean the collector. Combustion gas flow too low or too high. Check the detector gas flows. Column contaminated. Condition the column. Contaminated detector gas supply. Check the gas purity and install appropriate filters. Detector temperature higher than column maximum temperature.

Common Problems: Mobile Phase • The two chromatograms illustrate the effect of a small reduction (3 mM) in buffer concentration on the separation of six food additives: • Symptoms −Peaks shift −Loss of resolution • Causes −Mobile Phase buffer strength changes • Prevention . − Maintain buffer strength in aqueous phase.